(5:52:19 PM) Totensoldat: Hey Jeff
(5:53:07 PM) Totensoldat: So I was thinking about Olivia and look what I found.
(5:53:21 PM) Totensoldat: The mechanism by which it breaks peptide bonds involves deprotonation of Cys-25 by His-159. Asp-158 helps to orient the imidazole ring of His-159 to allow this deprotonation to take place. Cys-25 then performs a nucleophilic attack on the carbonyl carbon of a peptide backbone. This frees the amino terminal of the peptide, and forms a covalent acyl-enzyme intermediate. The enzyme is then deacylated by a water molecule, and releases the carboxy terminal portion of the peptide. In immunology, papain is known to cleave the Fc (crystallisable) portion of immunoglobulins (antibodies) from the Fab (antigen-binding) portion.
(5:54:03 PM) Totensoldat: A 10-minute treatment of small tissue pieces (less than 1 mm cubed) will allow papain to begin breaking down the extracellular matrix molecules holding the cells together. After 10 minutes, the tissue should be treated with a protease inhibitor solution to stop the protease action (if left untreated papain's activity will lead to complete lysis of the cells).
(5:54:13 PM) Totensoldat: Isn't it wonderful?
(5:57:21 PM) Totensoldat: Olivia would look so good with her protein broken down.
(6:02:34 PM) OmegaXRidley: I'm sorry but you've got the wrong person
(6:02:53 PM) Totensoldat: No I don't
(6:03:10 PM) OmegaXRidley: Well for one thing my name isn't Jeff
(6:03:23 PM) Totensoldat: Jeff do we have to play this game everytime?
(6:03:51 PM) Totensoldat: I get it you are trying to protect yourself from them, but no one even cares about what we are planning to do anyways.
(6:05:34 PM) OmegaXRidley: I see what you did there
(6:05:50 PM) Totensoldat: Why do you need to be so childish?
(6:05:58 PM) Totensoldat: memes, really?
(6:06:22 PM) OmegaXRidley: 1. Create AIM account
2. Message random AIM SN with Gackt in it
3. Discuss Olivia
4. ???
5. Profit
(10:34:47 PM) Totensoldat: Hello Jeff
(10:34:54 PM) Gacktinabottle: I'm not Jeff
(10:34:59 PM) Totensoldat: Yes you are
(10:36:03 PM) Totensoldat: so like I was saying, norcamphor is a camphor derivative with the three methyl groups replaced by hydrogen
(10:36:15 PM) Totensoldat: That's why Oliva would smell that way.
(10:36:20 PM) Gacktinabottle: Congrats on being an idiot.
(10:36:36 PM) Totensoldat: Jeff you needn't be so childish
(10:36:37 PM) Unable to send message: Not logged in
(6:06:31 PM) Totensoldat: Hoo
(6:06:33 PM) Totensoldat: damn
(6:06:43 PM) Totensoldat: Crackyyhouse is googleable now?
(6:06:50 PM) Totensoldat: shit that is never good
(6:07:03 PM) Totensoldat: Dueces
Not as planned
